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KMID : 0363720070400010027
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Korean Journal of Anatomy
2007 Volume.40 No. 1 p.27 ~ p.35
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Thioredoxin Peroxidases are Differentially Expressed in Developing Tooth Germs in Rats
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Kim Hyun-Jin
Kang Ji-Hye
Jeon Soo-Kyung
Jung Sun-Wa
Kim Min-Seok
Kim Won-Jae
Oh Won-Mann
Lee Eun-Joo
Jung Ji-Youn
Bae Choon-Sang
Kim Sun-Hun
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Abstract
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Thioredoxin peroxidase (prdx) is an antioxidant defense enzyme and is involved in cell differentiation. We elucidated the localization and expression of prdx in the rat tooth germ, which undergoes dynamic morphological changes during development. Immunohistochemical localization was performed using anti-prdx type I-VI antibodies, and their expression levels were determined at a transcriptional level. The six types of prdx showed varied immunoreactivities according to cellular stages in the developing tooth germ. Cells of the inner enamel epithelium and dental papilla showed relatively weak immunoreactivity, while ameloblasts and odontoblasts demonstrated strong reactivities to all six types of the enzyme. Of particular note, preameloblasts and preodontoblasts undergoing differentiation in the future cusp-forming region of the molar tooth (where the dentin first forms), showed stronger staining than those of the inner enamel epithelium and dental papilla cells in the same tooth germ of the bell stage. The expressions of types I, III and VI prdxs were not changed at the transcription level during tooth development, while those of types II, IV and V p increased in the hard tissue formation stage of tooth germs, compared with the cap or early bell stage. These results suggest that dental hard tissue cells might express prdx enzymes promiscuously and that production of these enzyme isoforms may be implicated in cell signaling between preameloblasts and preodontoblasts, besides serving as antioxidants.
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KEYWORD
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Prdx, Developing tooth, Oxidants, Odontoblasts, Ameloblasts
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